โจ๐๐๐บ๐๐๐บ๐๐๐บ
๐๐๐บ๐๐๐บ
๐๐บ๐
๐บ Is the bladder 100% sterile?:
================
๐ฐThe bladder was not originally tested because:
1๏ธโฃ It was considered to be sterile and there were complexities regarding sample collection.
2๏ธโฃ Culture techniques were developed for fast growing aerobic uropathogens.
Over the last couple years it has shown that a urinary microbiome exists and for most individuals it plays a protective role.
โโโ @Biochem_Lab โโโโข
๐ฐIt was noticed that patients with UTI symptoms, but negative standard urine cultures, contained slow growing organisms that required different growth conditions than those in standard method.
๐ฐLater, An enhanced quantitative urine culture (EQUC) protocol was developed.
๐ฐUnlike the standard urine culture protocol, which plates 1ฮผl urine on blood and MacConkey agar with incubation in air for 24 hours, EQUC plates a greater amount of urine (100ฮผl) on a variety of media with incubation under diverse atmospheric conditions for 48 hours.
The development of EQUC and other enhanced culture protocols showed that
The bladder is not 100% sterile.
https://telegram.me/joinchat/AAAAAECuqoTkgQkHpiHlGA
๐๐๐บ๐๐๐บ
๐๐บ๐
๐บ Is the bladder 100% sterile?:
================
๐ฐThe bladder was not originally tested because:
1๏ธโฃ It was considered to be sterile and there were complexities regarding sample collection.
2๏ธโฃ Culture techniques were developed for fast growing aerobic uropathogens.
Over the last couple years it has shown that a urinary microbiome exists and for most individuals it plays a protective role.
โโโ @Biochem_Lab โโโโข
๐ฐIt was noticed that patients with UTI symptoms, but negative standard urine cultures, contained slow growing organisms that required different growth conditions than those in standard method.
๐ฐLater, An enhanced quantitative urine culture (EQUC) protocol was developed.
๐ฐUnlike the standard urine culture protocol, which plates 1ฮผl urine on blood and MacConkey agar with incubation in air for 24 hours, EQUC plates a greater amount of urine (100ฮผl) on a variety of media with incubation under diverse atmospheric conditions for 48 hours.
The development of EQUC and other enhanced culture protocols showed that
The bladder is not 100% sterile.
https://telegram.me/joinchat/AAAAAECuqoTkgQkHpiHlGA