🧪 TISSUE PROCESSING EXIT EXAM MCQs
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1. The main aim of tissue processing is to:
A. Stain the tissue
B. Remove water and replace it with wax
C. Kill microorganisms
D. Increase tissue size
✔ Answer: B
2. Which step directly removes water from tissue?
A. Fixation
B. Clearing
C. Dehydration
D. Embedding
✔ Answer: C
3. The most commonly used dehydrating agent is:
A. Formalin
B. Alcohol
C. Xylene
D. Paraffin
✔ Answer: B
4. Which alcohol concentration is used last in dehydration?
A. 70%
B. 80%
C. 95%
D. Absolute alcohol
✔ Answer: D
5. The purpose of clearing is to:
A. Remove wax
B. Remove alcohol and prepare for wax
C. Remove fixative
D. Remove stain
✔ Answer: B
6. Which is the most common clearing agent?
A. Ethanol
B. Xylene
C. Formalin
D. Acetone
✔ Answer: B
7. Tissue becomes transparent during:
A. Fixation
B. Dehydration
C. Clearing
D. Embedding
✔ Answer: C
8. The temperature of paraffin wax during infiltration is usually:
A. 20–30°C
B. 30–40°C
C. 56–60°C
D. 80–100°C
✔ Answer: C
9. If wax temperature is too high, the tissue will:
A. Become soft
B. Become brittle and hard
C. Become watery
D. Increase in size
✔ Answer: B
10. Incomplete dehydration results in:
A. Perfect staining
B. Good infiltration
C. Poor infiltration
D. Faster embedding
✔ Answer: C
11. Correct sequence of tissue processing is:
A. Clearing > Dehydration> Embedding
B. Dehydration > Clearing> Infiltration > Embedding
C. Fixation > Embedding > Clearing
D. Clearing > Fixation > Embedding

✔ Answer: B

12. The final product of embedding is:
A. Slide
B. Tissue section
C. Paraffin block
D. Smear
✔ Answer: C
13. The most commonly used embedding medium is:
A. Gelatin
B. Paraffin wax
C. Agar
D. Resin glue
✔ Answer: B
14. Ideal thickness of tissue for processing is about:
A. 1–2 cm
B. 3–5 mm
C. 1 mm
D. 10 mm
✔ Answer: B
15. Incorrect orientation during embedding leads to:
A. Faster processing
B. Diagnostic error
C. Better staining
D. Tissue enlargement
✔ Answer: B
16. Which step makes tissue firm enough for microtome cutting?
A. Fixation
B. Clearing
C. Infiltration with wax
D. Washing
✔ Answer: C
17. Which factor most affects processing quality?
A. Slide color
B. Tissue thickness
C. Label size
D. Microscope type
✔ Answer: B
18. Rapid dehydration can cause:
A. Tissue swelling
B. Tissue shrinkage
C. Better staining
D. Wax melting
✔ Answer: B
19. Clearing agents must be miscible with:
A. Water only
B. Alcohol only
C. Alcohol and wax
D. Formalin
✔ Answer: C
20. Which step immediately precedes embedding?
A. Fixation
B. Dehydration
C. Clearing
D. Infiltration
✔ Answer: D


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@MedicallabAcademy
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